mPrP(3F4)-YEpFAT4
(Plasmid
#1144)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneYEpFAT4
- Backbone size w/o insert (bp) 8200
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePrP
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Alt namemouse prion protein
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SpeciesM. musculus (mouse)
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Insert Size (bp)629
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Mutation3F4 epitope (aa23-231); L108M, V111M
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GenBank IDNT_039207
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Entrez GenePrnp (a.k.a. CD230, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byunknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mPrP(3F4)-YEpFAT4 was a gift from Susan Lindquist (Addgene plasmid # 1144) -
For your References section:
De novo generation of a PrPSc-like conformation in living cells. Ma J, Lindquist S. Nat Cell Biol 1999 Oct;1(6):358-61. 10.1038/14053 PubMed 10559963