pBabe bleo human Frataxin
(Plasmid #11442)

• Depositing Lab: Ronald Kahn
• Publication: Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 97(22):12239-43.

Backbone

• Vector backbone: pBABE bleo
• Backbone manufacturer: pBABE-bleo/zeo is available at Addgene
• Backbone size w/o insert (bp): 4888
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Frataxin
• Alt name: FRDA
• Species: H. sapiens (human)
• Insert Size (bp): 650
• Entrez Gene: FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry