pAL3
(Plasmid
#114673)
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Purpose(Empty Backbone) T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114673 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonemosaic, has pBR322 ori
- Backbone size (bp) 5000
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Vector typeBacterial Expression
- Promoter T7
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Selectable markersccdB counter-selection
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Tag
/ Fusion Protein
- optional Ser-Gly-6His (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAL3 was a gift from William Studier (Addgene plasmid # 114673 ; http://n2t.net/addgene:114673 ; RRID:Addgene_114673)