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pET32a-HD16Q
(Plasmid #11487)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11487 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-32a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    huntingtin, exon 1, 16 glutamines
  • Alt name
    huntingtin (Huntington disease)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    279
  • Mutation
    huntingtin exon 1 with 16 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.
  • GenBank ID
    NM_002111
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tags / Fusion Proteins
    • Trx (N terminal on backbone)
    • His (C terminal on insert)
    • His (out of frame) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    R. Myers
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32a-HD16Q was a gift from Pamela Bjorkman (Addgene plasmid # 11487 ; http://n2t.net/addgene:11487 ; RRID:Addgene_11487)
  • For your References section:

    Inaugural Article: A linear lattice model for polyglutamine in CAG-expansion diseases. Bennett MJ, Huey-Tubman KE, Herr AB, West AP, Ross SA, Bjorkman PJ. Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9. 10.1073/pnas.182393899 PubMed 12193654