Addgene - pSV human p85 alpha (HA tag) Plasmid Data

Plasmid 11499: pSV human p85 alpha (HA tag)

Gene/insert name:
p85 alpha
Alt name:
PI3K regulatory subunit p85alpha
Insert size:
2200
Species:
H. sapiens (human)
Entrez Gene:
PIK3R1 (AGM7, GRB1, p85, p85-ALPHA)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Vector backbone:
pSV Sport
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3160
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
Unknown
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Ronald Kahn
Terms and Licenses:
MTA

Comments:

There is a short insert of aa (ENFLSCLPS) in the middle of the ORF compared to NCBI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Positive and negative roles of p85 alpha and p85 beta regulatory subunits of phosphoinositide 3-kinase in insulin signaling. Ueki et al (J Biol Chem. 2003 Nov 28. 278(48):48453-66. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11499" in your Materials and Methods section.