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pAcGP67A-PSMA
(Plasmid #11524)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11524 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcGP67A
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 9761
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    prostate-specific membrane antigen
  • Alt name
    PSMA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Mutation
    There is a short N-terminal truncation of this protein.
  • GenBank ID
    AAA60209
  • Entrez Gene
    FOLH1 (a.k.a. FGCP, FOLH, GCP2, GCPII, NAALAD1, PSM, PSMA, mGCP)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer 5' AAT TTAA AAT gAT AAC CAT CTC gC 3''
  • 3′ sequencing primer 5' gAA gCg ggT CCA AgT TTC CCT g 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Joseph Neale and Tomas Bzdega (Georgetown University, Washington, DC)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGP67A-PSMA was a gift from Pamela Bjorkman (Addgene plasmid # 11524 ; http://n2t.net/addgene:11524 ; RRID:Addgene_11524)
  • For your References section:

    Crystal structure of prostate-specific membrane antigen, a tumor marker and peptidase. Davis MI, Bennett MJ, Thomas LM, Bjorkman PJ. Proc Natl Acad Sci U S A. 2005 Apr 26. 102(17):5981-6. 10.1073/pnas.0502101102 PubMed 15837926