pZ M2rtTA_CAGG TetON-Sox10 with GFP
(Plasmid #115241)

• Purpose: RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)
• Depositing Lab: Catherine Verfaillie
• Publication: Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.

Backbone

• Vector backbone: pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2
• Backbone size w/o insert (bp): 9100
• Total vector size (bp): 10535
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SOX10
• Alt name: SRY-box 10
• Species: H. sapiens (human)
• Insert Size (bp): 1401
• GenBank ID: NM_006941.3
• Entrez Gene: SOX10 (a.k.a. DOM, PCWH, SOX-10, WS2E, WS4, WS4C)
• Tag / Fusion Protein:
  • GFP (separate polypeptide) (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: eGFP
• Alt name: GFP
• Insert Size (bp): 720

Resource Information

• Supplemental Documents:
  • pZ M2rtTA_CAGG TetON-Sox10 with GFP.ape
  • Restriction digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry