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Purpose(Empty Backbone) Generation of mariner-based transposon mutant libraries in Enterococcus faecium
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepZXL5
- Backbone size (bp) 11760
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Modifications to backboneMade two point mutations in the inverted terminal repeats of the himar1 transposon, introducing two MmeI sites
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Gentamicin, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsBoth chloramphenicol and gentamicin can be used as selective agents. Replicates in E. coli at 37°C, but is temperature sensitive in E. faecium (replicates at 30°C, but not at 37°C).
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGPA1 was a gift from Willem van Schaik (Addgene plasmid # 115476 ; http://n2t.net/addgene:115476 ; RRID:Addgene_115476) -
For your References section:
RNA-seq and Tn-seq reveal fitness determinants of vancomycin-resistant Enterococcus faecium during growth in human serum. Zhang X, de Maat V, Guzman Prieto AM, Prajsnar TK, Bayjanov JR, de Been M, Rogers MRC, Bonten MJM, Mesnage S, Willems RJL, van Schaik W. BMC Genomics. 2017 Nov 21;18(1):893. doi: 10.1186/s12864-017-4299-9. 10.1186/s12864-017-4299-9 [pii] PubMed 29162049
