PurposeExpresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||115778||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4200
- Total vector size (bp) 7300
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameAndrogen Receptor
SpeciesH. sapiens (human)
MutationR617M, K618M ,K632M, K633M, insertion of Q81 and Q82
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
Insert encodes 25 glutamines in the polymorphic region of the androgen receptor. Mutations are based on the numbering from the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-hAR-R617M,K618M,K632M,K633M was a gift from Elizabeth Wilson (Addgene plasmid # 115778 ; http://n2t.net/addgene:115778 ; RRID:Addgene_115778)
For your References section:A ligand-dependent bipartite nuclear targeting signal in the human androgen receptor. Requirement for the DNA-binding domain and modulation by NH2-terminal and carboxyl-terminal sequences. Zhou ZX, Sar M, Simental JA, Lane MV, Wilson EM. J Biol Chem. 1994 May 6;269(18):13115-23. PubMed 8175737