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Addgene

LV-Sox10MCS5-GFP
(Plasmid #115783)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115783 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    SIN18
  • Backbone size w/o insert (bp) 6378
  • Total vector size (bp) 7906
  • Modifications to backbone
    First, Sox10 multiple species conserved enhancer element 5 (MCS5, mouse CH15:79029307 CH15:79028761) coupled with a c-fos basal promoter was excised using Xho1/Age1 restriction enzyme digest from pXIG-MCS5Sox10-eGFP (plasmid described in Antonellis et al 2008). This was inserted into aMHC-eGFP-Res-Neo vector (Add gene # 21229) to replace the aMHC promoter. Rex-Neo selection cassette was removed by digesting the plasmid with Kpn1 and Spe1 plasmids, followed by blunting and blunt end ligation.
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    546
  • Promoter SOX10 Multiple Species Conserved enhancer element 5 coupled with cfos basal promoter
  • Tag / Fusion Protein
    • cfos basal promoter conjugated MCS5 promoter fused to eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site Age1 (unknown if destroyed)
  • 5′ sequencing primer AAAACTAGTctgggtccctccccacccag
  • 3′ sequencing primer AAAACTAGTttacttgtacagctcgtccatgcc
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The pXIG-Sox10-MCS5 plasmid contains Sox10 multiple species conserved enhancer element 5 (MCS5, mouse CH15:79029307CH15:79028761) coupled with a c-fos basal promoter and eGFP (as described in Antonellis, et al., 2008) was provided by A. McCallion of Johns Hopkins University. The single reporter lentivirus was generated by cloning Sox10 -MCS5:cfos enhancer/promoter cassette into the aMHCeGFP-Rex-Neo lentiviral reporter (AddGene, #21229) ( Kita-Matsuo et al., 2009 )

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV-Sox10MCS5-GFP was a gift from Fraser Sim (Addgene plasmid # 115783 ; http://n2t.net/addgene:115783 ; RRID:Addgene_115783)
  • For your References section:

    Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate. Pol SU, Lang JK, O'Bara MA, Cimato TR, McCallion AS, Sim FJ. Exp Neurol. 2013 Sep;247:694-702. doi: 10.1016/j.expneurol.2013.03.010. Epub 2013 Mar 16. 10.1016/j.expneurol.2013.03.010 PubMed 23507034