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Plasmid 1160: TM104b(G217S/T499I)
  • HSP104

  • 5799

  • 2850

  • S. cerevisiae (budding yeast)

  • M67479

  • HSP104 (YLL026W)

  • G217S/T499I

  • TMBS (CEN TRP1 MET3 promoter)
    (Search Vector Database)

  • Yeast Expression

  • 5000

  • BamHI

  • No

  • SacI

  • No

  • CGGGATGAACGACCAAACGC List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Susan Lindquist

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dominant gain-of-function mutations in Hsp104p reveal crucial roles for the middle region. Schirmer et al (Mol Biol Cell 2004 May;15(5):2061-72. Epub 2004 Feb 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1160" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with
18687: pRS414 GPD-Sis1
1159: TM104b(WT)