pcDNA3 proHB-EGF NC
(Plasmid #11605)

• Depositing Lab: Rosalyn Adam
• Publication: Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

Backbone

• Vector backbone: pcDNA3.1-myc/His(A)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HB-EGF
• Alt name: proHB-EGF
• Species: H. sapiens (human)
• Insert Size (bp): 667
• Mutation: Non-cleavable HB-EGF: Pro149 to Thr160 replaced with CD4 JM domain.
• Entrez Gene: HBEGF (a.k.a. DTR, DTS, DTSF, HEGFL)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We received a proHB-EGF plasmid incorporating the CD4 JM domain from Michael Klagsbrun, Vascular Biology Research program, Children's Hospital Boston. The original citation is Suzuki et al., 1997. J Biol Chem 272(50), 31730-737.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

JM domain of HB-EGF replaced with JM domain of CD4 (PVENPLYTYDHT replaced with KVLPTWSTPVQP). The original citation is Suzuki et al., 1997. J Biol Chem 272(50), 31730-737.