Addgene - pLVUHshp53-tTR-KRAB Plasmid Data

Plasmid 11654: pLVUHshp53-tTR-KRAB

Gene/insert name:
hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO
Alt name:
shRNA against p53
Alt name:
p53
Species:
H. sapiens (human)
Entrez Gene:
TP53 (BCC7, LFS1, P53, TRP53)
Vector backbone:
pLVU-tTR-KRAB
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
11579
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Patrick Aebischer
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.
You may also visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile tool for conditional gene expression and knockdown. Szulc et al (Nat Methods. 2006 Feb . 3(2):109-16. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11654" in your Materials and Methods section.