pMJA219= pcDNA3.1V5-completeTRABV-CMVp
(Plasmid #116878)

• Purpose: mammalian expression vector containing TCR sequences
• Depositing Lab: Marcos Alcocer
• Publication: Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-1104. doi: 10.1007/s10529-019-02713-2. Epub 2019 Jul 26.

Backbone

• Vector backbone: pcDNA3.1V5
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5203
• Total vector size (bp): 8506
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: complete TRAV10-TRBV25-CMVp rabbitpolyA
• Alt name: TCR alpha beta
• Alt name: bidirectional CMV Promoter
• Species: H. sapiens (human)
• Insert Size (bp): 3303
• GenBank ID: MH782476
• Entrez Gene: TRAV10 (a.k.a. TCRAV10S1, TCRAV24S1)
• Entrez Gene: TRBV25-1 (a.k.a. TCRBV11S1A1T, TCRBV25S1, TRBV251)
• Promoter: CMV Promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: Xbal (not destroyed)
• 5′ sequencing primer: not available
• 3′ sequencing primer: not available

Resource Information

• Supplemental Documents:
  • genebankfile.gb
• A portion of this plasmid was derived from a plasmid made by: Rui Wang, Ronja Pscheid, Ashfaq Ghumra, Marcos Alcocer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry