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V677 pDNR MCS BE lacZ alpha - Donor
(Plasmid #11694)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11694 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pDNR Dual
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 5177
  • Modifications to backbone
    Alternate MCS with BamHI, SalI, SmaI, HidIII, EcoRI sites and LacZ
  • Vector type
    Cre/Lox ; Donor vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Donor vector for the Creator Splice system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V677 pDNR MCS BE lacZ alpha - Donor was a gift from Tony Pawson (Addgene plasmid # 11694 ; http://n2t.net/addgene:11694 ; RRID:Addgene_11694)
  • For your References section:

    Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, Hardy WR, Pawson T, Morin GB. BMC Biotechnol. 2006 . 6():13. 10.1186/1472-6750-6-13 PubMed 16519801