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pET32-MCM2/7
(Plasmid #116949)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 116949 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-32 Ek/LIK
  • Total vector size (bp) 10930
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Human MCM7
  • Alt name
    minichromosome maintenance 7
  • Alt name
    CDC47
  • Alt name
    DNA replication licensing factor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2633
  • GenBank ID
    NP_005907.3 P33993.4
  • Entrez Gene
    MCM7 (a.k.a. CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104)
  • Tag / Fusion Protein
    • Thioredoxin, His6

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gacgacgacaagatggcactgaaggactacgcgctag
  • 3′ sequencing primer cgcgggcggccgtcagacaaaagtgatccgtgtccggg
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Human MCM2
  • Alt name
    minichromosome maintenance 2
  • Alt name
    DNA replication licensing factor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2735
  • GenBank ID
    NP_004517.2
  • Entrez Gene
    MCM2 (a.k.a. BM28, CCNL1, CDCL1, D3S3194, DFNA70, MITOTIN, cdc19)

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gcgggcccggcctccatggcggaatcatcggaatccttcacc
  • 3′ sequencing primer gaggagaagcccggtcagaactgctgcaggatcattttcc
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Juan Mendez CISC

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32-MCM2/7 was a gift from James Chong (Addgene plasmid # 116949 ; http://n2t.net/addgene:116949 ; RRID:Addgene_116949)
  • For your References section:

    DNA induces conformational changes in a recombinant human minichromosome maintenance complex. Hesketh EL, Parker-Manuel RP, Chaban Y, Satti R, Coverley D, Orlova EV, Chong JP. J Biol Chem. 2015 Mar 20;290(12):7973-9. doi: 10.1074/jbc.M114.622738. Epub 2015 Feb 3. 10.1074/jbc.M114.622738 PubMed 25648893