Addgene - V795 pDNR MCS ES - Donor Plasmid Data

Plasmid 11696: V795 pDNR MCS ES - Donor

Gene/insert name:
None
Vector backbone:
pDNR Dual
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Cre/Lox ; Donor vector
Backbone size (bp):
4777
Modifications to Backbone:
Alternate MCS with AscI, EcoRI, NotI, BamHI, SalI, PacI sites
5' sequencing primer:
M13 forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tony Pawson
Terms and Licenses:
MTA

Comments:

Donor vector for the Creator Splice system.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill et al (BMC Biotechnol. 2006 . 6():13. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11696" in your Materials and Methods section.