V179 pLP Triple-Flag - Acceptor
(Plasmid #11706)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    NA
  • Backbone size (bp) 4181
  • Vector type
    Mammalian Expression, Cre/Lox ; Acceptor vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Acceptor vector for the Creator Splice system.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V179 pLP Triple-Flag - Acceptor was a gift from Tony Pawson (Addgene plasmid # 11706)
  • For your References section:

    Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, Hardy WR, Pawson T, Morin GB. BMC Biotechnol. 2006 . 6():13. 10.1186/1472-6750-6-13 PubMed 16519801