Addgene - V181 pLPS 3' Triple-Flag - Acceptor Plasmid Data

Plasmid 11708: V181 pLPS 3' Triple-Flag - Acceptor

Gene/insert name:
None
Fusion protein or tag:
3xFLAG
Terminal:
C terminal on backbone
Vector backbone:
NA
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox ; Acceptor vector
Backbone size (bp):
4234
Modifications to Backbone:
Splice for 3' Tags
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tony Pawson
Terms and Licenses:
MTA

Comments:

Acceptor vector for the Creator Splice system.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill et al (BMC Biotechnol. 2006 . 6():13. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11708" in your Materials and Methods section.