Addgene - V621 pGEX-4T-LP - Acceptor Plasmid Data

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Plasmid 11715: V621 pGEX-4T-LP - Acceptor

Gene/insert name:
None
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Vector backbone:
NA
(Search Vector Database)
Vector type:
Bacterial Expression, Cre/Lox
Vector type:
Acceptor vector
Backbone size w/o insert:
5127
5' sequencing primer:
pGEX3 List of Sequencing Primers
Bacterial resistance:
Ampicillin
Growth strain:
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tony Pawson
Terms and Licenses:
MTA

Comments:

Acceptor vector for the Creator Splice system.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill et al (BMC Biotechnol. 2006 . 6():13. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11715" in your Materials and Methods section.