pCMV5-Smad7-HA
(Plasmid #11733)

• Depositing Lab: Jeff Wrana
• Publication: Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMAD7
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• GenBank ID: NM_005904
• Entrez Gene: SMAD7 (a.k.a. CRCS3, MADH7, MADH8)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA