pMD19_STARR-seq_plants
(Plasmid #117379)

• Purpose: (Empty Backbone) STARR-seq vector with 35s core promoter for transient expression in plant protoplasts
• Depositing Lab: Xiaoyu Zhang
• Publication: Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s41477-019-0547-0. doi: 10.1038/s41477-019-0547-0.

Backbone

• Vector backbone: T-Vector pMD19 (Simple)
• Backbone manufacturer: Clontech
• Backbone size (bp): 6332
• Modifications to backbone: T-Vector pMD19 (Simple) was digested with EcoR V and inserts were ligated into the backbone.
• Vector type: Plant Expression
• Promoter: 35s core promoter
• Tag / Fusion Protein:
  • GFP derived from pMDC107 (N terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • pMD19_STARR-seq_plants.gb
• A portion of this plasmid was derived from a plasmid made by: The GFP sequence was derived from pMDC107. The sequence between attP1 and attP2 was derived from pDONR 207.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is designed for transient transfection of protoplasts. It has only been validated in Zea mays mesophyll protoplasts. The region containing the ccdB suicide gene is dropped out by a double-digest with SacI and KpnI and the large fragment is recombined with the DNA insert library.