Addgene - pCMV5B-FLAG-SARA Plasmid Data

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Plasmid 11738: pCMV5B-FLAG-SARA

Gene/insert name:
SARA
Insert size:
4400
Species:
H. sapiens (human)
GenBank ID:
AF104304
Entrez Gene:
ZFYVE9 (MADHIP, NSP, SARA, SMADIP)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pCMV5B
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
SmaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: SARA, a FYVE domain protein that recruits Smad2 to the TGFbeta receptor. Tsukazaki et al (Cell. 1998 Dec 11. 95(6):779-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11738" in your Materials and Methods section.