Addgene - pCMV5-ALK2 Q207D Plasmid Data

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Plasmid 11740: pCMV5-ALK2 Q207D

Gene/insert name:
ALK2
Insert size:
1500
Species:
H. sapiens (human)
GenBank ID:
NM_001105
Entrez Gene:
ACVR1 (ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI)
Mutation:
Q207D: activated receptor
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
SalI
Site destroyed during cloning:
Yes
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance:
Ampicillin
Growth strain:
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2. Macias-Silva et al (J Biol Chem. 1998 Oct 2. 273(40):25628-36. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11740" in your Materials and Methods section.