Addgene - pCMV5B-Flag-Smurf1 C699A Plasmid Data

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Plasmid 11753: pCMV5B-Flag-Smurf1 C699A

Gene/insert name:
SMURF1
Insert size:
2300
Species:
H. sapiens (human)
GenBank ID:
NM_020429
Entrez Gene:
SMURF1 (KIAA1625)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Mutation:
C699A: catalytically inactive
Vector backbone:
pCMV5B
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
ClaI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Zhu et al (Nature. 1999 Aug 12. 400(6745):687-93. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11753" in your Materials and Methods section.