Addgene - pCMV5-ALK6-HA Plasmid Data

Gene/insert name:
ALK6
Insert size:
1900
Species:
M. musculus (mouse)
Entrez Gene:
Bmpr1b (AI385617, ALK-6, AV355320, Acvrlk6, Alk6, BMPR-1B, BMPR-IB, CFK-43a, SKR6)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4700
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jeff Wrana
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2. Macias-Silva et al (J Biol Chem. 1998 Oct 2. 273(40):25628-36. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11769" in your Materials and Methods section.