pCS2+_Flag-myc-NES-Tir1
(Plasmid #117717)

• Purpose: mRNA synthesis of Flag-myc-NES-TIR1 for injection
• Depositing Lab: Joerg Mansfeld
• Publication: Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.

Backbone

• Vector backbone: pCS2+
• Backbone manufacturer: RZPD
• Backbone size w/o insert (bp): 4089
• Total vector size (bp): 5925
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRANSPORT INHIBITOR RESPONSE 1
• Alt name: TIR1
• Species: Oryza sativa
• Mutation: codon optimized TIR1 for murine expression
• Promoter: SP6
• Tag / Fusion Protein:
  • Flag-Myc-NES (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ATTTAGGTGACACTATAG

Resource Information

• Supplemental Documents:
  • Addgene_pCS2_Flag-Myc_NES-TIR1.gb
• A portion of this plasmid was derived from a plasmid made by: We received the backbone containing codon-optimized TIR1 from Francis Stewart (TU Dresden, BIOTEC Institute, Tatzberg 47-49, 01307 Dresden, Germany
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The codon-optimized TIR1 is described in:

Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).