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pPRISM-Cre-cmlc2-TagBFP
(Plasmid #117792)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 117792 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pK
  • Backbone manufacturer
    Karl J. Clark
  • Backbone size w/o insert (bp) 2856
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre
  • Alt name
    Cre Recombinase
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    4339

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aaaagcaagaaagaaaactagagtgg
  • 3′ sequencing primer atggctcataacaccccttg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at <www.genesculpt.org/gtaghd/>;. May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPRISM-Cre-cmlc2-TagBFP was a gift from Jeffrey Essner (Addgene plasmid # 117792 ; http://n2t.net/addgene:117792 ; RRID:Addgene_117792)
  • For your References section:

    GeneWeld: a method for efficient targeted integration directed by short homology. Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Weiss TJ, Torrie ME, Vollbrecht MK, Lan M, McKeighan KC, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity B, Solin SL, Carlson DF, Dobbs DL, McGrail M, Essner JJ.. bioRxiv 431627 10.1101/431627