tetO-Bcl2-IRES-tdtomato(TET005)
(Plasmid #117857)

• Purpose: This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.
• Depositing Lab: C. Ron Yu
• Publication: Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6. doi: 10.1016/j.neuron.2018.09.051. Epub 2018 Oct 25.

Backbone

• Vector backbone: tetO-Kir2.1-IRES-tauLacZ
• Backbone manufacturer: Yu et al.
• Backbone size w/o insert (bp): 6044
• Total vector size (bp): 6755
• Modifications to backbone: tau-LacZ was replaced with tdTomato.
• Vector type: Mammalian Expression ; tTA/tetO

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bcl2
• Alt name: B-cell lymphoma 2
• Species: M. musculus (mouse)
• Insert Size (bp): 711
• Entrez Gene: Bcl2 (a.k.a. Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)
• Promoter: tetO

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTAAAACGACGGCCAG
• 3′ sequencing primer: CAGGAAACAGCTATGAC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry