Addgene - p426 25Q GPD Plasmid Data

Plasmid 1181: p426 25Q GPD

Gene/insert name:
htt 25Q
Insert size:
250
Species:
H. sapiens (human)
GenBank ID:
NM_002111
Entrez Gene:
HTT (HD, IT15)
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Vector backbone:
p426 GPD
(Search Vector Database)
Vector type:
Yeast Expression
5' sequencing primer:
M13R List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Sequence: View sequences (1)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Comments:

N terminal region of Ht with polyQ repeat length of 25 fused to GFP.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins. Krobitsch et al (Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1589-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1181" in your Materials and Methods section.

Price: $65.00

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