Addgene - pcDNA3-Casp3-myc Plasmid Data

Plasmid 11813: pcDNA3-Casp3-myc

Gene/insert name:
Caspase 3
Insert size:
983
Species:
H. sapiens (human)
Entrez Gene:
CASP3 (CPP32, CPP32B, SCA-1)
Fusion protein or tag:
Myc
Terminal:
C terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
Don't know
Site destroyed during cloning:
Unknown
Cloning site 3':
Don't know
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Principal Investigator:
Guy Salvesen
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Biochemical characteristics of caspases-3, -6, -7, and -8. Stennicke et al (J Biol Chem. 1997 Oct 10. 272(41):25719-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11813" in your Materials and Methods section.