FLAG-OTUB1 T134R,C91S-pcDNA3.1
(Plasmid #118212)

• Purpose: Expresses FLAG-OTUB1 T134R,C91S in mammalian cells
• Depositing Lab: Cynthia Wolberger
• Publication: Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-18295. doi: 10.1074/jbc.RA118.004677. Epub 2018 Oct 3.

Backbone

• Vector backbone: pcDNA3.1
• Total vector size (bp): 6253
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OTUB1
• Alt name: Otubain-1
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Mutation: changed cysteine 91 to serine and threonine 134 to arginine
• Tag / Fusion Protein:
  • 3x FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry