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pAAV-CamKII-GFP-P2A-bReaches-minWPRE
(Plasmid #118276)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118276 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CW3SL
  • Backbone manufacturer
    Bong-Kiun Kaang
  • Backbone size w/o insert (bp) 3771
  • Total vector size (bp) 5591
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    710
  • GenBank ID
  • Promoter CamK II
  • Tag / Fusion Protein
    • P2A (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer GTTCTCCGTTTGCACTCAGGA
  • 3′ sequencing primer GGGTTCTCCTCCACGTCTC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    bReaCHES
  • Species
    Synthetic
  • Insert Size (bp)
    915
  • GenBank ID
  • Promoter CamK II

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CGGAGCTACTAACTTCAGCC
  • 3′ sequencing primer CGTATCCACATAGCGTAAAAGGAGCAA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CamKII-GFP-P2A-bReaches-minWPRE was a gift from Dietmar Schmitz (Addgene plasmid # 118276 ; http://n2t.net/addgene:118276 ; RRID:Addgene_118276)
  • For your References section:

    Potassium channel-based optogenetic silencing. Bernal Sierra YA, Rost BR, Pofahl M, Fernandes AM, Kopton RA, Moser S, Holtkamp D, Masala N, Beed P, Tukker JJ, Oldani S, Bonigk W, Kohl P, Baier H, Schneider-Warme F, Hegemann P, Beck H, Seifert R, Schmitz D. Nat Commun. 2018 Nov 5;9(1):4611. doi: 10.1038/s41467-018-07038-8. 10.1038/s41467-018-07038-8 PubMed 30397200