PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118595||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepT2/SVNeo, a gift from Perry Hackett (Addgene 26553 – Cui et al., 2002)
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NruI (destroyed during cloning)
- 3′ cloning site ApaI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Please also cite the following publication:
Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007 Jul 16;178(2):257-68. PubMed PMID: 17635936; PubMed Central PMCID: PMC2064445.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CDK1as_T2A_puromycin was a gift from William Earnshaw (Addgene plasmid # 118595 ; http://n2t.net/addgene:118595 ; RRID:Addgene_118595)
For your References section:An intrinsic S/G2 checkpoint enforced by ATR. Saldivar JC, Hamperl S, Bocek MJ, Chung M, Bass TE, Cisneros-Soberanis F, Samejima K, Xie L, Paulson JR, Earnshaw WC, Cortez D, Meyer T, Cimprich KA. Science. 2018 Aug 24;361(6404):806-810. doi: 10.1126/science.aap9346. 10.1126/science.aap9346 PubMed 30139873