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CDK1as_T2A_Zeocin
(Plasmid #118596)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 118596 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT2/SVNeo, a gift from Perry Hackett (Addgene 26553 – Cui et al., 2002)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDK1
  • Species
    X. laevis (frog)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nru1 (destroyed during cloning)
  • 3′ cloning site Apa1 (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please also cite the following publication:
Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007 Jul 16;178(2):257-68. PubMed PMID: 17635936; PubMed Central PMCID: PMC2064445.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CDK1as_T2A_Zeocin was a gift from William Earnshaw (Addgene plasmid # 118596 ; http://n2t.net/addgene:118596 ; RRID:Addgene_118596)
  • For your References section:

    An intrinsic S/G2 checkpoint enforced by ATR. Saldivar JC, Hamperl S, Bocek MJ, Chung M, Bass TE, Cisneros-Soberanis F, Samejima K, Xie L, Paulson JR, Earnshaw WC, Cortez D, Meyer T, Cimprich KA. Science. 2018 Aug 24;361(6404):806-810. doi: 10.1126/science.aap9346. 10.1126/science.aap9346 PubMed 30139873