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p426 25Q GAL
(Plasmid #1187)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1187 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p426 GAL
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    htt 25Q
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    250
  • GenBank ID
    NM_002111
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N terminal region of Ht with polyQ repeat length of 25 fused to GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p426 25Q GAL was a gift from Susan Lindquist (Addgene plasmid # 1187 ; http://n2t.net/addgene:1187 ; RRID:Addgene_1187)
  • For your References section:

    Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins. Krobitsch S, Lindquist S. Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1589-94. 10.1073/pnas.97.4.1589 PubMed 10677504