pGTMV
(Plasmid #118755)

• Purpose: Binary plasmid with a tobacco mosaic virus infectious clone
• Depositing Lab: Jose-Antonio Daros
• Publication: Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10.1111/pbi.13038.

Backbone

• Vector backbone: pCLEAN-G181
• Total vector size (bp): 9669
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow Escherichia coli for 36 h at 37ºC in kanamycin plates. Grow Agrobacterium tumefaciens for 4 days at 28ºC in plates with rifampicin, tetracycline and kanamycin. Note that replication in A. tumefaciens requieres a pSoup-derivative as a helper plasmid.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tobacco mosaic virus
• Alt name: TMV
• Species: Tobacco mosaic virus
• Promoter: Cauliflower mosaic virus 35S

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry