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pISlin41m(s) (lin-41 microRNA mut reporter)
(Plasmid #11907)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11907 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIS0
  • Backbone manufacturer
    Available at Addgene (plasmid 12178)
  • Backbone size w/o insert (bp) 5264
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lin-41 miRNA target sequence mut
  • Alt name
    lin-41
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    103
  • Mutation
    point mutations in miRNA complementary site
  • Entrez Gene
    lin-41 (a.k.a. CELE_C12C8.3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site XbaI/NheI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Used as a control for testing effects of lin-41 microRNA (let-7).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISlin41m(s) (lin-41 microRNA mut reporter) was a gift from David Bartel (Addgene plasmid # 11907 ; http://n2t.net/addgene:11907 ; RRID:Addgene_11907)
  • For your References section:

    Prediction of mammalian microRNA targets. Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB. Cell. 2003 Dec 26. 115(7):787-98. 10.1016/S0092-8674(03)01018-3 PubMed 14697198