Plasmid 11913: pCerulean-VSVG

• Gene/insert name: VSVG
• Alt name: Vesicular stomatitus virus G protein
• Insert size: 1500
• Mutation: F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M153T, V163A, A206K relative the wild type GFP sequence. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used.
• Vector backbone: pECFP-N1
  (Search Vector Database)
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Backbone size w/o insert (bp): 4733
• Cloning site 5': BglII
• Site destroyed during cloning: No
• Cloning site 3': EcoRI
• Site destroyed during cloning: No
• 5' sequencing primer: CMV-F
• Bacterial resistance(s) Kanamycin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: Unknown
• Selectable markers: Neomycin
• Sequence: View sequences (2)
• Principal Investigator: Jennifer Lippincott-Schwartz
• Terms and Licenses: MTA

Comments: 

Cerulean ws developed by Piston lab. Reference is Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004) Nat Biotechnol 22, 445-9. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEGFP-N1 plasmid from Clontech has been disrupted.

Article: ER-to-Golgi transport visualized in living cells. Presley et al (Nature. 1997 Sep 4. 389(6646):81-5. PubMed)