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Browse > Jennifer Lippincott-Schwartz > Presley et al > pVenus-VSVG
Plasmid 11914: pVenus-VSVG

  • VSVG

  • Vesicular stomatitus virus G protein

  • 1500

  • F46L, F64L, M153T, V163A, S175G, T203Y, A206K relative the wild type GFP sequence. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used.

  • pEYFP-N1
    (Search Vector Database)

  • Clontech

  • Mammalian Expression

  • 4733

  • BglII

  • No

  • EcoRI

  • No

  • CMV-F List of Sequencing Primers

  • Kanamycin

  • DH5alpha

  • 37

  • Unknown

  • Neomycin

  • View sequences (2)

  • Jennifer Lippincott-Schwartz

  • MTA

Comments: 

The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEYFP-N1 plasmid from Clontech has been disrupted. Reference for the A206K mutation is Zacharias, D. A., Violin, J. D., Newton, A. C., and Tsien, R. Y. (2002) Science 296, 913-6. Reference for Venus is Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: ER-to-Golgi transport visualized in living cells. Presley et al (Nature. 1997 Sep 4. 389(6646):81-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11914" in your Materials and Methods section.

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