Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

LZF97_hSyn-spine-jRGECO1a
(Plasmid #119198)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119198 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    fsyn
  • Backbone size w/o insert (bp) 8447
  • Total vector size (bp) 11903
  • Modifications to backbone
    Insertion of ZFn (CCR5-L) binding site and Kozak.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L)
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    3456
  • GenBank ID
    NM_013070.1
  • Promoter Human Synapsin 1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGACCATCTGCGCTG
  • 3′ sequencing primer GCATTAAAGCAGCGTATC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LZF97_hSyn-spine-jRGECO1a was a gift from Don Arnold (Addgene plasmid # 119198 ; http://n2t.net/addgene:119198 ; RRID:Addgene_119198)
  • For your References section:

    All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition. Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE. Nat Methods. 2018 Oct;15(10):823-831. doi: 10.1038/s41592-018-0142-8. Epub 2018 Oct 1. 10.1038/s41592-018-0142-8 PubMed 30275587