pGEX-4T-1_RAF1 (51-131)
(Plasmid
#119218)
-
Purposepurification of GST-RAF1 RBD from E. coli
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepGEX-4T-1
- Backbone size w/o insert (bp) 4969
- Total vector size (bp) 5212
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsOther strains can also be used.
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameRAF1 aa 51-131
-
Alt nameRAF1 RBD
-
SpeciesH. sapiens (human)
-
Insert Size (bp)246
-
GenBank ID
-
Entrez GeneRAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGEX-4T-1_RAF1 (51-131) was a gift from Helen Morrison (Addgene plasmid # 119218 ; http://n2t.net/addgene:119218 ; RRID:Addgene_119218) -
For your References section:
Inhibition of RAS activation due to a homozygous ezrin variant in patients with profound intellectual disability. Riecken LB, Tawamie H, Dornblut C, Buchert R, Ismayel A, Schulz A, Schumacher J, Sticht H, Pohl KJ, Cui Y, Reis A, Morrison H, Abou Jamra R. Hum Mutat. 2015 Feb;36(2):270-8. doi: 10.1002/humu.22737. 10.1002/humu.22737 PubMed 25504542
