Addgene - Venus-GalT Plasmid Data

Plasmid 11931: Venus-GalT

Gene/insert name:
GalT
Insert size:
180
Species:
H. sapiens (human)
Entrez Gene:
GALT (hCG_2040046)
Mutation:
Amino acids 1-60 of the human galactosyltransferase. Venus has the A206K mutation to disrupt self-association.
Vector backbone:
pEYFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4733
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Forward List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Jennifer Lippincott-Schwartz
Terms and Licenses:
MTA

Comments:

Venus was developed by Miyawaki and colleagues. The reference is Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90. The EYFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Diffusional mobility of Golgi proteins in membranes of living cells. Cole et al (Science. 1996 Aug 9. 273(5276):797-801. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11931" in your Materials and Methods section.