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pBS594 promoterless EGFPcre
(Plasmid #11956)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11956 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-cre
  • Alt name
    cre
  • Species
    bacteriophage P1
  • Insert Size (bp)
    1800
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

Resource Information

Depositor Comments

pBS594 differs from pBS592 in having the MT-I A(n) instead of the GCSF A(n). MT-I A(n) is the mouse metallothionein MT-I region including the polyadenylation site and several introns. pBS592 carries the EGFPcre fusion gene in a promoterless vector with several available restriction sites. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression. Two related constructs are the pBS597 with a tet-controlled promoter and the vector pBS598 having constitutive high level expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS594 promoterless EGFPcre was a gift from Brian Sauer (Addgene plasmid # 11956 ; http://n2t.net/addgene:11956 ; RRID:Addgene_11956)
  • For your References section:

    GFPcre fusion vectors with enhanced expression. Le Y, Miller JL, Sauer B. Anal Biochem. 1999 Jun 1. 270(2):334-6. 10.1006/abio.1999.4110 PubMed 10334853