Addgene - pGEX-NEMO Plasmid Data

Gene/insert name:
NEMO
Alt name:
IKKgamma
Insert size:
1290
Species:
H. sapiens (human)
GenBank ID:
NM_003639
Entrez Gene:
IKBKG (AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IP, IP1, IP2, IPD2, NEMO)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Vector backbone:
pGEX4T1
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert:
4900
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX 5' primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jon Ashwell
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: NEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Wu et al (Nat Cell Biol. 2006 Apr . 8(4):398-406. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11965" in your Materials and Methods section.