pGEMT-NRTN
(Plasmid #119896)

• Purpose: Mouse NRTN In situ hybridization probe
• Depositing Lab: Wenqin Luo
• Publication: Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554/eLife.06828.

Backbone

• Vector backbone: pGEM-T
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Vector type: in situ hybridization probe

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: neurturin probe
• Alt name: Nrtn
• Species: M. musculus (mouse)
• Entrez Gene: Nrtn (a.k.a. NTN)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be used to generate an in situ hybridization antisense probe for mouse NRTN, using T7 to transcribe the ~1kb probe.