pDEST-tetOx224_PhiC31attB_FRT-PGKneo-FRT
(Plasmid #119902)

• Purpose: PhiC31 integration plasmid for tetO array with Neo selection cassette
• Depositing Lab: Orion Weiner
• Publication: Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.

Backbone

• Vector backbone: pDEST-PhiC31attB-FRT-PGK-neo-FRT
• Backbone size w/o insert (bp): 5464
• Total vector size (bp): 14953
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tetOx224 array
• Species: Synthetic
• Insert Size (bp): 9489

Resource Information

• Supplemental Documents:
  • 163_pDEST_tetOx224_PhiC31attB_FRT_PGKneo_FRT.gb
  • cuO and tetO array genome integration protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PhiC31 integrase expression plasmid available on addGene (pPGKPhiC31obpA: #13795).

The repetitive sequences in this plasmid can be unstable while propagated in bacteria. It is recommended that the repetitive insert be verified by restriction digest and repeat counts be viewed as an approximation.

Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.