pRK5_tAKAR4α
(Plasmid
#119919)
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PurposeMammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametAKAR4α
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5_tAKAR4α was a gift from Haining Zhong (Addgene plasmid # 119919 ; http://n2t.net/addgene:119919 ; RRID:Addgene_119919) -
For your References section:
A Highly Sensitive A-Kinase Activity Reporter for Imaging Neuromodulatory Events in Awake Mice. Ma L, Jongbloets BC, Xiong WH, Melander JB, Qin M, Lameyer TJ, Harrison MF, Zemelman BV, Mao T, Zhong H. Neuron. 2018 Aug 22;99(4):665-679.e5. doi: 10.1016/j.neuron.2018.07.020. Epub 2018 Aug 9. 10.1016/j.neuron.2018.07.020 PubMed 30100256
