pMVA2
(Plasmid #119951)

• Purpose: Expresses recombinant MVA pathway in Escherichia coli
• Depositing Lab: Nigel Scrutton
• Publication: Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398.

Backbone

• Vector backbone: pBbA1k-RFP
• Backbone manufacturer: Lee, Taek Soon, et al. "BglBrick vectors and datasheets: a synthetic biology platform for gene expression." Journal of biologica
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mvaE
• Species: Enterococcus faecalis
• Insert Size (bp): 2412

Gene/Insert 2

• Gene/Insert name: mvaS
• Species: Enterococcus faecalis
• Mutation: A110G

Gene/Insert 3

• Gene/Insert name: mvaK1
• Species: Streptococcus pneumoniae

Gene/Insert 4

• Gene/Insert name: mvaK2
• Species: Streptococcus pneumoniae

Gene/Insert 5

• Gene/Insert name: mvaD
• Species: Streptococcus pneumoniae

Gene/Insert 6

• Gene/Insert name: idi
• Species: Escherichia coli

Resource Information

• Supplemental Documents:
  • pMVA2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry