Addgene - pAG228 TNKS2 3'UTR mut Plasmid Data

Plasmid 12031: pAG228 TNKS2 3'UTR mut

Gene/insert name:
TNKS2 3'UTR mut
Alt name:
TNKS2
Insert size:
845
Species:
H. sapiens (human)
GenBank ID:
NM_025235
Entrez Gene:
TNKS2 (PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, pART6)
Mutation:
Mutations in microRNA recognition sites (seed matches): 1760-1765 and 2254-2259.
Vector backbone:
pIS2
(Search Vector Database)
Backbone manufacturer:
pIS2 derived from pRL-SV40 (Promega)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
3700
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
No
5' sequencing primer:
EBV rev primer (GTGGTTTGTCCAAACTCATC) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

TNKS2 3'UTR mutant in renilla luciferase reporter plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Farh et al (Science. 2005 Dec 16. 310(5755):1817-21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12031" in your Materials and Methods section.